Light stimulates growth of proteorhodopsin-containing marine Flavobacteria

Supplementary Figure 1. Proteorhodopsin amino acid sequence alignment. Multiple alignment of the predicted aminoacid sequences of PR in Dokdonia sp. MED134 and Polaribacter sp. MED152 compared to tentative Bacteroidetes orthologs and SAR86 and P. ubique sequences. Predicted transmembrane regions are marked by boxes with solid lines. Gray shading indicates conserved amino acid positions. Key amino acids for PR functionality are marked by colours: Asp97 and Glu108 in yellow, Met, Leu or Gln at position 105 in red, and Lys230 in light blue (SAR86 eBAC31A08 numbering). Amino acid positions predicted to be part of the retinal binding pocket are marked by arrows. Accession numbers and phylogenetic relationships of the sequences are presented in Fig. 2. Amino acid sequences were aligned using Clustal W. Predicted transmembrane regions were identified using TMpred, based on a statistical analysis of TMbase. SUPPLEMENTARY INFORMATION doi: 10.1038/nature05381